Article 5419

Title of the article

THE USE OF HORMONAL STIMULATION FOR REPRODUCTION OF THE KARELIN’S NEWT, TRITURUS KARELINII IN LABORATORY CONDITIONS 

Authors

Kidov Artem Aleksandrovich, Candidate of biological sciences, associate professor, sub-department of zoology, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49, Timiryazevskaya street, Moscow, Russia), E-mail: kidov_a@mail.ru
Shimanskaya Elizaveta Aleksandrovna, Master degree student, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49, Timiryazevskaya street, Moscow, Russia), E-mail: kara-59@mail.ru
Askenderov Azim Daniyalovich, Candidate of biological sciences, senior lecturer, sub-department of zoology and physiology, Daghestan State University (43A, M. Gadzhieva street, Makhachkala, Russia), E-mail: askenderov@mail.ru
Nemyko Elena Aleksandrovna, Engineer, sub-department of zoology, Russian State Agrarian University – Moscow Timiryazev Agrarian Academy (49, Timiryazevskaya street, Moscow, Russia), E-mail: nemyko_e@mail.ru 

Index UDK

591.16:597.8 

DOI

10.21685/2307-9150-2019-4-5 

Abstract

Background. The Karelin’s newt, Triturus karelinii – Crimean and Caucasian species, inhabiting mountain and foothill forests. This newt included in the Red Data Books of the Russian Federation and a number of North Caucasus regions. The North-Eastern periphery of the species distribution is located in Dagestan. In this Republic, the species is not recorded in specially protected natural territories and is the most vulnerable. A current conservation method of the Karelin’s newt in Dagestan is captive breeding and introduction into new ponds within the native areal. Purpose of this work: research of reproductive characteristics in the Karelin’s newt in captivity with use of hormonal stimulation of breeding.
Materials and methods. 10 pairs of newts from Tabasaran administrative district of Dagestan were used in the work. Per pair of animals was kept in plastic containers filled with 3 liters of water. Newts were fed through the day, larvae of chironomids (bloodworms). Reproduction was stimulated by injection of surfagon. To females three times were injected into the belly through the day with 12,5 mg of hormone on per newt. Males were stimulated by the same dose of hormone, but only once. We noted the mating behavior of males and cases of oviposition. Eggs from the containers seized on a daily basis. Eggs, pre-larvae and larvae were measured by standard methods.
Results. Of the ten females, only eight bred. The first eggs from different females were provided in the period from 19 to 29 January 2 to 12 days after the last hormonal injection. Oviposition by females of the Karelin’s newt continued 81–133 days. Oviposition by females of the Karelin’s newt continued 81–133 days. Calculated mean daily fertility was equal to 0,6–1,3 eggs. The highest fertility in females was observed in the first month of reproduction (January), and then sharply decreased by May. The largest diameter of laid eggs varied from 3,6 to 5,5 mm and the smallest diameter from 2,4 mm to 3,8 mm. Incubation of eggs at water temperature 16–19 °C lasted 15 days. The total length of the body and tail at the pre-larvae was 9,0–9,8 mm. The duration of the period from the exit from the egg to the beginning of exogenous feeding at a water temperature of 17,0–20,5 °C was 4–7 days. The total length of the larvae at the beginning of feeding was 9,7–10,2 mm. The last cases of oviposition in different females were observed in the period from April 9 to June 1.
Conclusions. The authors note that the use of hormonal injections is advisable for reproduction stimulate of the Karelin’s newt. This method allows you to force the start of oviposition. 

Key words

the Karelin’s newt, Triturus karelinii, reproductive biology, Daghestan, hormonal stimulation, zooculture 

 

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Дата создания: 22.01.2020 16:11
Дата обновления: 10.02.2020 12:01